Direct Shoot Organogenesis from Lycium chinense Miller Leaf Explants and Assessment of Genetic Stability Using ISSR Markers

An efficient in vitro direct shoot regeneration system has been described for Lycium chinense Miller using leaf explants. Influence of various parameters such as growth regulator concentration, explant type, effect basal salt Murashige and Skoog (1962) medium (MS), Schenk Hildebrandt (1972) (SH), Gamborg et al. (1968) (B5), carbon sources (sucrose, maltose, fructose) on the regenerating shoots studied. Micromorphological studies genetic fidelity regenerated were assessed compared with those donor plants. Among different concentrations plant (PGRs) tested, MS supplemented lower concentration 6-benzylaminopurine (BAP) (0.5 mgL?1) thidiazuron (TDZ) increased frequency shoot. Comparatively, indole-3-butyric acid (IBA) was more effective root system. A higher number formation (6.67 ± 1.25) observed when rooting comprised half-strength salts 3% sucrose. The surviving plantlets gradually transferred to greenhouse natural soil. More than 90% survived matured within 85 days. Similarity band patterns produced by inter simple sequence repeat (ISSR) primers confirmed stability uniformity between present optimized may be useful mass propagation improving traits L. chinense.